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Image Search Results
Journal: Medical Microbiology and Immunology
Article Title: Quantitative assay to analyze neutralization and inhibition of authentic Middle East respiratory syndrome coronavirus
doi: 10.1007/s00430-024-00789-w
Figure Lengend Snippet: Comparison of MERS-CoV MNA to VNT 100 . a Exemplary inhibition curves of the antibody m336, a negative human serum, and the MERS-CoV WHO international standard for immunoglobulins (WHO IS, human serum) in the microneutralization assay (MNA). The logarithmic concentration of m336 or the logarithmic serum dilution is plotted against the foci reduction in % of the virus only control (VOC). R 2 values from the 4PL regression analysis and the calculated IC 50 values are noted on the graph. The dotted line visualizes the 50% inhibition compared to VOC. Repeated analysis of m336 ( b ) and WHO IS ( c ) in the MNA and the VNT 100 to calculate IC 50 and IC 80 values (MNA) or IC 100 values (VNT 100 ). d Correlation of the IC 50 values generated with the MNA and the IC 100 values of the VNT 100 . The line reflects the best-fit linear relationship between the variables, and the dotted lines represent 95% confidence intervals. p and r values reflect two-tailed Spearman’s rank-correlation tests. The LLODs of each test are visualized by the gray lines
Article Snippet: These values were used to interpolate 50% or 80% serum neutralization titer (NT 50 or NT 80 ) or inhibitory concentration (IC 50 or IC 80 ) by
Techniques: Comparison, Inhibition, Microneutralization Assay, Concentration Assay, Virus, Control, Generated, Two Tailed Test
Journal: Medical Microbiology and Immunology
Article Title: Quantitative assay to analyze neutralization and inhibition of authentic Middle East respiratory syndrome coronavirus
doi: 10.1007/s00430-024-00789-w
Figure Lengend Snippet: Inhibition of MERS-CoV by the pan-CoV inhibitor EK1C4. The inhibition curves of the antibody m336 ( a ) and EK1C4 ( b ) in the MNA. The logarithmic concentration of m336 or the molarity of EK1C4 is plotted against the foci reduction in % of the virus only control (VOC). R 2 values from the 4PL regression analysis and the calculated IC 50 values are noted on the graph
Article Snippet: These values were used to interpolate 50% or 80% serum neutralization titer (NT 50 or NT 80 ) or inhibitory concentration (IC 50 or IC 80 ) by
Techniques: Inhibition, Concentration Assay, Virus, Control